梭梭幼苗根系分泌物提取方法的研究Study on the Extracts Methods of the Root Exudates from the Seedling of Haloxylon ammodendron(C.A.Mey.) Bunge
张汝民,张丹,陈宏伟,白静,高岩
摘要(Abstract)：
为了探讨梭梭根系分泌物中的化学成分,本文采用水浸提取和循环灌溉两种方法对梭梭幼苗根系分泌物进行收集和提取,结合气相色谱-质谱联用分析技术,分析梭梭幼苗根系分泌物中组分和含量的差异。实验结果表明:循环灌溉收集的梭梭幼苗根系分泌物中检测到35种化合物,包括酚醌类、醛酮类、醇类、酸类、脂类和胺类化合物;硅烷化处理后,主要增加部分强极性有机酸,如苯甲酸。水浸提取梭梭幼苗根系分泌物的中性相组分共检测出27种化合物,但未检测到酚醌类化合物;碱性相组分共检测出23种化合物,醛酮类化合物种类减少,烷烃类化合物种类增加;酸性相组分中仅检测到10种化合物,以醇类化合物为主,占检出化合物总量的72.62%。收集梭梭幼苗根系分泌物循环灌溉是一种有效的方法。
关键词(KeyWords)： 梭梭;根系分泌物;水浸提取;循环灌溉;GC/MS分析
基金项目(Foundation): 国家自然科学基金项目(30360086);; 内蒙古自然科学基金项目(200208020502)资助
作者(Author): 张汝民,张丹,陈宏伟,白静,高岩
参考文献(References)：
- [1]Bais HP,Park SW,Weir TL,Callaway RM,Vivanco JM.How plants communicate using the underground information superhighway[J].Trends in Plant Science,2004,9(1):26-32.
- [2]Bais HP,Weir TL,Perry LG,Gilroy S,Vivanco JM.The Role of Root Exudates in Rhizosphere Interactions with Plants and Other Or-ganisms[J].Annul Review of Plant Biology,2006,57:233-66.
- [3]Chang M,Lynn DG.The haustorium and the chemistry of host recognition in parasitic angiosperms[J].Journal of Chemical Ecology,1986,12(2):561-579.
- [4]Estabrook EM and Yoder JI.Plant-plant communications:Rhizosphere signaling between parasitic angiosperms and their hosts[J].Plant Physiology,1998,116:1-7.
- [5]Keyes WJ,Taylor JV,Apkarian RP,and Lynn DG.Dancing Together.Social Controls in Parasitic Plant Development[J].Plant Physi-ology,2001,127:1508-1512.
- [6]Matvienko M,Wojtowicz A,Wrobel R,Jamison DS,Goldwasser Y and Yoder JI.Quinone oxidoreductase message levels are differenti-ally regulated in parasitic and non-parasitic plants exposed to all elopathic quinones[J].The Plant Journal,2001,25(4):375-387.
- [7]Matusova R,Rani K,Verstappen FWA,Franssen MCR,Beale MH,and Bouwmeester HJ.The Strigolactone Germination Stimulantsof the Plant-Parasitic Striga and Orobanche spp[J].Are Derived from the Carotenoid Pathway.Plant Physiology,2005,139(2):920-934.
- [8]Netzly DH,Butler LG.Root of sorghum exude hydrophobic droplets containing biologically active components[J].Crop Science.1986,26:775-778.
- [9]Truong R,Nava AJ,Nava A,and Yoder JI.Chemical signaling between plants:mechanistic similarities between allelopathy and hostplant recognition by parasitic Angiosperms.In:Dicke,M.and Takken,W.eds.Chemical ecology:from genes to ecosystem.Springer,Berlin.2005,55-69.
- [10]Yoder JI.Parasitic plant responses to host plant signals:a model for subterranean plant-plant interactions[J].Curr.Opin.Plant Biol.1999,2:65-70.
- [11]韩丽梅,沈其荣,等.大豆地上部水浸液的化感作用及化感物质的鉴定[J].生态学报,2002,22(9):1425-1430.
- [12]何海斌,陈祥旭,等.化感水稻PI312777苗期根系分泌物中化学成分分析[J].应用生态学报,2005,16(12):2383-2388.
- [13]胡飞,孔垂华.寄生植物对寄主植物的化学识别[J].生态学报,2003,23(5):965-971.
- [14]李天然.寄生被子植物的种子生理及其与寄主的相互关系[J].植物生理学通讯,1996,32(6):450-457.