四种牧草根际固氮细菌的分离及固氮活性的研究Isolation of Nitrogen Fixing Bacteria From Rhizosphere of Leymus Chinensis and Investigation on Their Growth Conditions
郑红丽,李少红,樊明寿,乌兰巴特尔,王曙光
摘要(Abstract):
为了获得禾本科牧草根际土壤固氮菌分布的基本情况及优势菌株,于牧草旺盛生长季节,从内蒙古锡林郭勒天然草地的羊草、大针茅、冰草、糙隐子草根际土壤、原土体以及根内进行了固氮细菌的分离,并统计了数量分布。结果表明,内蒙古锡林郭勒草地土壤中普遍存在固氮菌,而且禾本科牧草根际显著高于自由土体中的固氮菌数量。其中大针茅根际不仅固氮菌数量多而且菌株固氮酶活性高。另外,本研究还通过乙炔还原法筛选出四种不同牧草根际固氮能力最强的固氮细菌各1株,并观察了其形态学特征和菌落特征,这为进一步的理论研究以及开发利用奠定了基础。
关键词(KeyWords): 固氮细菌;分离;形态学特征
基金项目(Foundation): 国家自然科学基金项目(36560031);; 内蒙古自然科学基金项目(200208020302)资助
作者(Author): 郑红丽,李少红,樊明寿,乌兰巴特尔,王曙光
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